Alkaline agarose gel electrophoresis buffer

For 10x solution: Add 50 ml of 10 N NaOH and 20 ml of 0.5 M EDTA (pH 8.0) to 800 ml of H₂O and then adjust the final volume to 1 liter. Dilute the 10x alkaline agarose gel electrophoresis buffer with H₂O to generate a 1x working solution immediately before use. Use the same stock of 10x alkaline agarose gel electrophoresis buffer to prepare the alkaline agarose gel and the 1x working solution of alkaline electrophoresis buffer.