Recipe

HEPES-buffered saline (16-3)

 
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21 mM HEPES

0.7 mM Na2HPO4

6 mM dextrose

5 mM KCl

137 mM NaCl

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Adjust the pH of the solution to 7.10. Sterilize the solution by filtration and store frozen in 25-50-ml aliquots. Thaw a fresh aliquot before each transfection and check the pH of a small volume. Readjust pH is necessary to 7.10.

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  1. doi:10.1101/pdb.rec8788 Cold Spring Harb Protoc 2006: pdb.rec8788-
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