Recipe

Tissue resuspension buffer

 
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5 mM HEPES-KOH (pH 7.9)

0.5 mM phenylmethylsulfonyl fluoride

26% (v/v) glycerol

0.5 mM dithiothreitol

1.5 mM MgCl2

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Store the buffer at 0°C until needed.

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  1. doi:10.1101/pdb.rec8808 Cold Spring Harb Protoc 2006: pdb.rec8808-
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