Recipe

Buffer A

 
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50 mM Tris-Cl (pH 7.9)

3 mM MgCl2

0.2 mM phenylmethylsulfonyl fluoride

100 mM NaCl

1 mM dithiothreitol

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Store Buffer A at 4°C. Add dithiothreitol and PMSF to Buffer A just before use.

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  1. doi:10.1101/pdb.rec8811 Cold Spring Harb Protoc 2006: pdb.rec8811-
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