Recipe

Lysis buffer (17-3)

 
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0.05% (v/v) saponin

200 mM β-mercaptoethanol

100 mM KCl

50 mM Tris-Cl (pH 7.9)

50% (v/v) glycerol

5 mM MgCl2

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Store lysis buffer at 4°C. Add β-mercaptoethanol to the lysis buffer just before use.

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  1. doi:10.1101/pdb.rec8813 Cold Spring Harb Protoc 2006: pdb.rec8813-
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