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0.05% (v/v) saponin
200 mM β-mercaptoethanol
100 mM KCl
50 mM Tris-Cl (pH 7.9)
50% (v/v) glycerol
5 mM MgCl2
Store lysis buffer at 4°C. Add β-mercaptoethanol to the lysis buffer just before use.
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