Recipe

DNase I solution

Dilute a preparation of RNase-free DNase I to a concentration of 10 units/μl using DNase dilution buffer. Store the solution at -20°C. One unit is defined as the amount of DNase that will cause a change in A260 of 0.001/minute/ml of a reaction using a calf thymus DNA substrate.

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  1. doi:10.1101/pdb.rec8816 Cold Spring Harb Protoc 2006: pdb.rec8816-
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