Recipe

Lysis buffer (17-4)

 
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10 mM Tris-Cl (pH 8.4 at 4°C)

0.14 M NaCl

1.5 mM MgCl2

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Store the buffer at 4°C.

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  1. doi:10.1101/pdb.rec8822 Cold Spring Harb Protoc 2006: pdb.rec8822-
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