Reaction buffer (17-4)
For a 2x buffer: Store the buffer at 4°C. Just before use, add 5 μl of 1 M dithiothreitol, 100 μl of 100 mM ATP, 10 μl of 100 mM CTP, and 10 μl of 100 mM GTP to 1 ml of 2x reaction buffer.
For a 2x buffer: Store the buffer at 4°C. Just before use, add 5 μl of 1 M dithiothreitol, 100 μl of 100 mM ATP, 10 μl of 100 mM CTP, and 10 μl of 100 mM GTP to 1 ml of 2x reaction buffer.