Recipe

Reaction buffer (17-4)

0.3 M KCl

5 mM MgCl2

10 mM Tris-Cl (pH 8.0)

For a 2x buffer: Store the buffer at 4°C. Just before use, add 5 μl of 1 M dithiothreitol, 100 μl of 100 mM ATP, 10 μl of 100 mM CTP, and 10 μl of 100 mM GTP to 1 ml of 2x reaction buffer.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec8828 Cold Spring Harb Protoc 2006: pdb.rec8828-

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Alert me when Updates/Comments are published

Share

Navigate This Article

  1. Top