Recipe

Ethyl acetate

Ethyl acetate (CH2COOCH2CH3) is an organic solvent used in thin-layer chromatographic assays of CAT activity. Mix 1 part ethyl acetate with 19 parts chloroform to make a solvent that separates acetylated and nonacetylated forms of chloramphenicol on silica gel plates. Ethyl acetate is an extremely flammable (flash point = -4°C) and volatile ester with a scent of ripe fruit.

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  1. doi:10.1101/pdb.rec8831 Cold Spring Harb Protoc 2006: pdb.rec8831-
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