Recipe

Luciferase cell lysis buffer

 
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25 mM glycylglycine (pH 7.8)

1% (v/v) Triton X-100

15 mM MgSO4

4 mM EGTA

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Just before use, add dithiothreitol from a 1 M stock solution to a final concentration of 1 mM. Approximately 1 ml of cell lysis buffer is needed per 100-mm dish of cells to be assayed.

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  1. doi:10.1101/pdb.rec8835 Cold Spring Harb Protoc 2006: pdb.rec8835-
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