Recipe

GST basic buffer

 
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0.1% Nonidet P-40

14 mM β-mercaptoethanol

1 mM dithiothreitol

20 mM HEPES (pH 7.5)

50 mM KCl

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  1. doi:10.1101/pdb.rec8849 Cold Spring Harb Protoc 2006: pdb.rec8849-
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