Recipe

GST blocking buffer

 
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20 mM HEPES (pH 7.5)

50 mM KCl

14 mM β-mercaptoethanol

1 mM dithiothreitol

0.1% Nonidet P-40

5% nonfat dry milk

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  1. doi:10.1101/pdb.rec8850 Cold Spring Harb Protoc 2006: pdb.rec8850-
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