Recipe

Prehybridization/Hybridization for Hybridization in Phosphate-SDSBuffer

Use an electrophoresis grade of bovine serum albumin. No blocking agents or hybridization rate enhancers are required with this particular prehybridization/hybridization solution.

*0.5 M phosphate buffer is 134 g of Na2HPO4•7H2O, 4 ml of 85% H3PO4 (concentrated phosphoric acid), H2O to 1 liter.

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  1. doi:10.1101/pdb.rec8874 Cold Spring Harb Protoc 2006: pdb.rec8874-
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