Recipe

2x Bacteriophage T4 DNA ligase mixture

1 M Tris-Cl (pH 7.6), 1.0 μl

100 mM MgCl2, 2.0 μl

200 mM dithiothreitol, 1.0 μl

10 mM ATP, 1.0 μl

Bacteriophage T4 DNA ligase, 1 Weiss unit

H20, 4.5 μl

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  1. doi:10.1101/pdb.rec8875 Cold Spring Harb Protoc 2006: pdb.rec8875-
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