Recipe

Bacteriophage T4 DNA polymerase repair buffer

 
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90 mM (NH4)2SO4

0.33 M Tris-Cl (pH 8.3)

33 mM MgCl2

50 mM β-mercaptoethanol

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For a 5x buffer. Store the buffer in small aliquots at -20°C.

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  1. doi:10.1101/pdb.rec8901 Cold Spring Harb Protoc 2006: pdb.rec8901-
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