Recipe

Sequenase reaction buffer with MgCl2

 
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200 mM Tris-Cl (pH 7.5)

125 mM NaCl

100 mM MgCl2

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For a 5x buffer. Store at -20°C.

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  1. doi:10.1101/pdb.rec8963 Cold Spring Harb Protoc 2006: pdb.rec8963-
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