Recipe

Synthesis buffer

 
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100 mM Tris-Cl (pH 7.5)

dTTP, dATP, dCTP, and dGTP, each at a concentration of 5 mM

10 mM ATP

20 mM dithiothreitol

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For a 10x buffer.

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  1. doi:10.1101/pdb.rec9017 Cold Spring Harb Protoc 2006: pdb.rec9017-
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