Recipe

Exonuclease III buffer

660 mM Tris-Cl (pH 8.0)

66 mM MgCl2

100 mM β-mercaptoethanol

Add β-mercaptoethanol to the 10x stock just before use.

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  1. doi:10.1101/pdb.rec9032 Cold Spring Harb Protoc 2006: pdb.rec9032-

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