Guanidine Denaturation Solution
Dilute 10x binding buffer with 5 volumes of distilled H2O. The resulting solution (2x binding buffer) is then added to the appropriate amount of solid guanidine HCl to make a 6 M solution. When the guanidine HCl has completely dissolved, adjust the concentration of the binding buffer to 1x with distilled H2O and add dithiothreitol to a final concentration of 1 mM. About 15 ml of full-strength (6 M) Guanidine Denaturation Solution is needed for each 82-mm filter to be screened, or 25 ml for each 138-mm filter.
