Recipe

Screening buffer

 
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1x binding buffer

0.25% (w/v) nonfat dry milk

10 μg/ml denatured Salmon Sperm DNA

or herring sperm DNA

1 mM dithiothreitol

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About 10 ml of screening buffer is needed for each 82-mm filter or 25 ml for each 138-mm filter.

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  1. doi:10.1101/pdb.rec9067 Cold Spring Harb Protoc 2006: pdb.rec9067-
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