Recipe

Coomassie Brilliant Blue solution

Dissolve 0.25 g of Coomassie Brilliant Blue R-250 in 90 ml of methanol:H2O (1:1, v/v) and 10 ml of glacial acetic acid. Filter the solution through a Whatman No. 1 filter to remove any particulate matter. Store at room temperature.

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  1. doi:10.1101/pdb.rec9077 Cold Spring Harb Protoc 2006: pdb.rec9077-
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