MOPS salts

400 mM 3-(N-morpholino)propanesulfonic acid (pH 7.4) (MOPS)

40 mM Tricine (pH 7.4)

0.1 mM FeSO₄•7H₂O

95 mM NH₄Cl

2.8 mM K₂SO₄

5 μM CaCl₂•2H₂O

5.3 mM MgCl₂

0.5 M NaCl

(Used in induction medium) Dissolve in a final volume of 1 liter, and sterilize by filtration. Add 10 μl of micronutrients per liter before use.