Recipe

Inclusion-body solubilization buffer I

 
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8 M urea

0.1 M PMSF

100 mM NaCl

1 mM EDTA (pH 8.0)

50 mM Tris-Cl (pH 8.0)

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Prepare the buffer fresh just before use.

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  1. doi:10.1101/pdb.rec9110 Cold Spring Harb Protoc 2006: pdb.rec9110-
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