Recipe

Cell homogenization buffer

1.5 mM MgCl2

0.5 mM dithiothreitol

0.5 mM phenylmethylsulfonyl fluoride

10 mM HEPES-KOH (pH 7.9)

10 mM KCl

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  1. doi:10.1101/pdb.rec9123 Cold Spring Harb Protoc 2006: pdb.rec9123-

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