Recipe

Cell resuspension buffer (17-1)

 
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40 mM HEPES-KOH (pH 7.9)

0.4 M KCl

0.1 mM phenylmethylsulfonyl fluoride

0.1% (w/v) aprotinin

10% (v/v) glycerol

1 mM dithiothreitol

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Store the buffer at 0°C until needed.

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  1. doi:10.1101/pdb.rec9124 Cold Spring Harb Protoc 2006: pdb.rec9124-
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