Recipe

Mg2+ solution

0.1 M MgCl2

4.5 M β-mercaptoethanol

For a 100x solution: Just before use, add an appropriate amount of β-mercaptoethanol from a 14.7 M stock.

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  1. doi:10.1101/pdb.rec9146 Cold Spring Harb Protoc 2006: pdb.rec9146-

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