Recipe

Cell lysis buffer (15-6)

30 mM Tris-Cl (pH 7.1)

0.1 mM EDTA

20% (w/v) sucrose

(Approx. 150 ml)

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  1. doi:10.1101/pdb.rec9231 Cold Spring Harb Protoc 2006: pdb.rec9231-

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