RP-HPLC Solvent A (14 mM) (aqueous 0.1% v/v trifluoroacetic acid)
Add 1.0 ml of neat TFA (using a Gilson pipette, or equivalent) to 1 liter of H2O in a glass-stoppered measuring cylinder, mix thoroughly, and then pour into a clean liquid chromatograph/HPLC reservoir bottle(extensively washed with Milli-Q H2O). It is good practice to set aside a 1-liter cylinder to be used only for mixing TFA and H2O. Use HPLC-grade acetonitrile, TFA, and H2O.
