Recipe

HEMGN buffer

 
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25 mM HEPES (K+, pH 7.6)

0.1 mM EDTA

2.5 mM MgCl2

10% glycerol

0.025% NP-40

1 mM dithiothreitol (DTT)

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Add DTT fresh immediately before use. Store the buffer at 4°°C

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  1. doi:10.1101/pdb.rec9830 Cold Spring Harb Protoc 2006: pdb.rec9830-
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