Recipe

EcoRI Methylase Buffer

 
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10 mM EDTA

80 µM S-adenosylmethionine80 µM S-adenosylmethionine80 µM S-adenosylmethionine80 µM S-adenosylmethionine80 µM S-adenosylmethionine

50 mM NaCl

50 mM Tris-Cl (pH 8.0)

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Store the 1x buffer in small aliquots at -20°C.

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  1. doi:10.1101/pdb.rec9958 Cold Spring Harb Protoc 2006: pdb.rec9958-
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