Recipe

Dye-ligand affinity column washing buffer

The ideal buffer to use will depend on the binding conditions of the protein of interest. Good choices to consider are Tris-HCl (pH 7.0-7.5) containing 20-100 mM KCl, or 20 mM MOPS/NaOH.

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  1. doi:10.1101/pdb.rec9982 Cold Spring Harb Protoc 2006: pdb.rec9982-
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