Table 1. Preparation of elution buffers containing varying concentrations of imidazole
| Final concentration of imidazole (mM) | Volume (ml) of | |
|---|---|---|
| IMAC Buffer Pa | IMAC high imidazole buffer | |
| 20 | 9.6 | 0.4 |
| 40 | 9.2 | 0.8 |
| 60 | 8.8 | 1.2 |
| 100 | 8 | 2 |
| 300 | 4 | 6 |
| 500 | 0 | 10 |
a IMAC Buffer P is 20 mM sodium phosphate (pH 7.4) containing 0.5 M NaCl.
Table 1.
Preparation of elution buffers containing varying concentrations of imidazole
