Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents

Ed Harlow; David Lane

doi:10.1101/pdb.prot4527

Protocol

Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents

Ed Harlow and
David Lane

This protocol was adapted from “Staining Tissues,” Chapter 6, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

INTRODUCTION

Fluorochrome-linked reagents should be used when high resolution is needed or if two antigens need to be localized simultaneously. Because of the thickness of fly specimens, detection requires access to a confocal microscope.