Protocol

Drying SDS-Polyacrylamide Gels

This protocol was adapted from “Commonly Used Techniques in Molecular Cloning,” Appendix 8, in Molecular Cloning, Volume 3, 3rd edition (eds. Sambrook and Russell). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001.

INTRODUCTION

This protocol describes a method for drying SDS-polyacrylamide gels. Gels containing proteins radiolabeled with 35S-labeled amino acids must be dried before autoradiographic images can be obtained. Nonradioactive gels can also be preserved by drying.

A more recent Protocol discussing this method is available

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