Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
This protocol was adapted from “Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports,” contributed by Ronald Frank and Stefan Dübel, Chapter 31, in Protein-Protein Interactions, 2nd edition (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
The following protocol describes the synthesis of short linear peptides, or peptide pools, on modified cellulose membranes, and the detection of their protein-binding partners. Peptides are synthesized from their carboxyl termini using Fmoc-amino acid derivatives. After completion of the synthesis and cleavage of all side-chain-protecting groups, the peptide array on the membrane is incubated with the potential interaction partners to identify their target sequences.
