ChIP cell lysis buffer

5 mM PIPES (pH 8)

85 mM KCl

0.5% NP-40

The above solution is stable at room temperature.

Before use, add:

1 mM PMSF

Proteinase inhibitors, each final concentration 2 μg/ml:

1. leupeptin

2. aprotinin

3. pepstatin

Keep final solution on ice.