Fixation solution
11% formaldehyde (from a 37% stock equilibrated with methanol)
0.5 mM EGTA
50 mM HEPES (pH 8)
100 mM PMSF (phenylmethylsulfonyl fluoride) in isopropanol
Prepare immediately before use.
11% formaldehyde (from a 37% stock equilibrated with methanol)
0.5 mM EGTA
50 mM HEPES (pH 8)
100 mM PMSF (phenylmethylsulfonyl fluoride) in isopropanol
Prepare immediately before use.