Recipe

Ligase buffer

12.5 mM MgCl2

25 mM dithiothreitol (DTT)

1.25 mM ATP

50 mM Tris-Cl (pH 7.6)

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  1. doi:10.1101/pdb.rec10614 Cold Spring Harb Protoc 2006: pdb.rec10614-
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