Radioisotopic Labeling of Eukaryotic Cell Proteins Using 35S
This protocol was adapted from “Preparative 2D Gel Electrophoresis with Immobilized pH Gradients,” Chapter 4 in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.INTRODUCTION
Radioisotope labeling of proteins is an important technique in expression proteomics studies for detecting low-abundance proteins. The procedure given below describes 35S labeling. After the labeling procedure described here, cell lysates can be prepared for analysis by 2D gel electrophoresis.
