Recipe

Trypsin stock solution

1 mg/ml trypsin

1 mM HCl

20 mM CaCl2

Use fresh or divide the solution into small aliquots (~50 μl) and store frozen at -20ºC. A stock solution may be thawed and refrozen a few times, but for consistent results, thaw only once.

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  1. doi:10.1101/pdb.rec10581 Cold Spring Harb Protoc 2006: pdb.rec10581-
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