Recipe

Triton/glycerol lysis buffer

1% Triton X-100

10% glycerol

150 mM NaCl

20 mM Tris (pH 7.5)

2 mM EDTA

Immediately before use, supplement lysis buffer with:

1 mM PMSF

10 μg/ml aprotinin

10 μg/ml leupeptin

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  1. doi:10.1101/pdb.rec10593 Cold Spring Harb Protoc 2006: pdb.rec10593-
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