Recipe

Rehydration solution

8 M urea

1.5% (w/v) CHAPS

Dissolve 48 g of urea and 1.5 g of CHAPS in water to a final volume of 100 ml.

Divide the rehydration solution into 2.5- to 5-ml aliquots, and freeze them at -20ºC.

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  1. doi:10.1101/pdb.rec10651 Cold Spring Harb Protoc 2006: pdb.rec10651-
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