Recipe

Acrylamide mix (30%)

Acrylamide

N,N′-methylene-bisacrylamide

To prepare the 30% acrylamide mix, combine 30% (w/v) acrylamide and 1.0% N,N′-methylene-bisacrylamide in H2O. The final mixture is 30%T and 3.3%C.

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  1. doi:10.1101/pdb.rec10663 Cold Spring Harb Protoc 2006: pdb.rec10663-
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