Table 1. Recommended electrophoresis conditions for second-dimension vertical gels
| Step | Current (mA/gel) | Duration (hr:min) | ||
| Mini vertical system | ||||
| 1.5-mm-thick gel | 1 | 15 | 0:15 | |
| 2 | 30 | 1:30a | ||
| 1.0-mm-thick gel | 1 | 10 | 0:15 | |
| 2 | 20 | 1:30a | ||
| Standard vertical system | ||||
| 1.5-mm-thick gel | 1 | 15 | 0:15 | |
| 2 | 45 | 5:00-6:00a | ||
| 1.0-mm-thick gel | 1 | 15 | 0:15 | |
| 2 | 45 | 5:00-6:00a | ||
| aTime shown here is only an estimate. Electrophoresis should be stopped when the dye is 0.5-1.0 cm from the bottom of the gel. | ||||
