Table 1. Recommended electrophoresis conditions for IPG gels on flatbed IEF systems
| Step | IPG length (cm) | Voltage | Current (mA) | Power (W) | Mode | Duration (hr:min) | Volt-hours |
| 1 | 7,11,13,18, 24 | 200 | 2 | 5 | Step | 0:01 | 1 |
| 2 | 7,11,13,18, 24 | 3500 | 2 | 5 | Gradienta | 1:30 | 2800 |
| 3 | 7 | 3500 | 2 | 5 | Step | 0:55-1:30 | 3200-5200 |
| 3 | 11 | 3500 | 2 | 5 | Step | 2:20-3:30 | 8100-12,100 |
| 3 | 13 | 3500 | 2 | 5 | Step | 3:45-4:20 | 13,100-18,100 |
| 3 | 18 | 3500 | 2 | 5 | Step | 5:40-7:40 | 23,000-30,000 |
| 3 | 24 | 3500 | 2 | 5 | Step | 14:30-25:45 | 50,000-90,000 |
| aDuring Step 2, the voltage should rise from 200 V to 3500 V over the 1:30 time period. | |||||||
