Table 1. A Y1H scoring system
| Scoring of | Scoring criterion | Low score | High score |
| DNA bait | lacZ self-active? | High = 0 | Low, medium = 1 |
| HIS3 self-active? | ≥ 80 mM 3AT = 0 | <80 mM 3AT = 1 | |
| INTERACTOR | Predicted TF or novel? | Novel = 0 | Predicted TF = 1 |
| INTERACTION | Times found | Found once with one library = 0(go to Library scoring 1) | Found multiple times = 2(go to Library scoring 2) |
| Library scoring 1 | AD-TF library = 0 | AD-cDNA library = 1 | |
| Library scoring 2 | Multiple times from one library = 1 | Found with both AD-TF and AD-cDNA libraries = 2 |
