| Temperature |
Step |
Power (W/gel) |
Duration (hr:min) |
| 25ºC |
1 |
5 |
0:15 |
|
2 |
15 |
6:00a |
| 20ºC |
1 |
5 |
0:15 |
|
2 |
10 |
8:00a |
| 15ºC |
1 |
5 |
0:45 |
|
2 |
6.6 |
10:00-12:00a |
| aTime shown here is only an estimate. Electrophoresis should be stopped when the dye is 0.5-1.0 cm from the bottom of the gel.
Values given here are based on the limit of the cooling system’s capacity at the given temperature and are for 12 gels. Power
levels can be increased if running fewer than 12 gels, which will result in reduced run times. These values are optimized
for the Ettan DALT II system (Amersham Biosciences).
|
