Table

Table 2. Some FRET symbols and their interpretation

Symbol Filter set Fluorochromes present Meaning
Dd Donor Donor Signal from a donor-only specimen using the donor filter cube
Fd FRET Donor Signal from a donor-only specimen using the FRET filter set
Ad Acceptor Donor Signal from a donor-only specimen using the acceptor filter set
Da Donor Acceptor Signal from an acceptor-only specimen using the donor filter cube
Fa FRET Acceptor Signal from an acceptor-only specimen using the FRET filter set
Aa Acceptor Acceptor Signal from an acceptor-only specimen using the acceptor filter set
Df Donor Donor and acceptor Signal from a FRET specimen using the donor filter cube
Ff FRET Donor and acceptor Signal from a FRET specimen using the FRET filter set
Af Acceptor Donor and acceptor Signal from a FRET specimen using the acceptor filter set
Adapted from Gordon et al. 1998 and reprinted with permission from the Biophysical Society © 1998.
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  1. doi:10.1101/pdb.tab294581 Cold Spring Harb Protoc 2006: pdb.tab294581-
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